Please use this identifier to cite or link to this item: http://dspace.agu.edu.vn:8080/handle/AGU_Library/7083
Nhan đề: Water metagenomic analysis reveals low bacterial diversity and the presence of antimicrobial residues and resistance genes in a river containing wastewater from backyard aquacultures in the Mekong Delta, Vietnam.
Tác giả: Nakayama, Tatsuya
Hoa, Tran Thi Tuyet
Harada, Kazuo
Warisaya, Minae
Asayama, Megumi
Hinenoya, Atsushi
Lee, Joon Won
Phuong, Nguyen Thanh
Phu, Tran Minh
Từ khoá: Water microbiota
Aquaculture
Antimicrobial residue
Antimicrobial resistance gene
Waste water
Năm xuất bản: Mar-2017
Tùng thư/Số báo cáo: Environmental Pollution;Volume 222, Page 294-306
Tóm tắt: The environmental pathways for the dissemination of antibiotic resistance have recently received increased attention. Aquatic environments act as reservoirs or sources of antimicrobial-resistant bacteria, antimicrobial residues, and antimicrobial resistance genes (ARGs). Therefore, it is imperative to identify the role of polluted water in the dissemination of antimicrobial resistance. The aim of this study was to evaluate the antimicrobial residues, ARGs, and microbiota in the freshwater systems of the Mekong Delta. We selected 12 freshwater sites from aquacultures and rivers in Can Tho, Vietnam and analyzed them for 45 antimicrobial residues and 8 ARGs by LC/MS/MS and real-time PCR, respectively. A 16S rDNA-based metagenomic analysis was conducted to characterize the water microbiota. Residues of sulfamethoxazole (10/12) and sulfadimidine (7/12) were widely detected, together with the sulfa-resistance genes sul1 (11/12) and sul2 (9/12). Additionally, sulfamethoxazole residues and the β-lactamase-resistance gene blaCTX-M-1 were detected in eight freshwater systems (8/12), suggesting that these freshwater systems may have been polluted by human activity. The metagenomic analysis showed that all the tested freshwater systems contained the phyla Proteobacteria, Actinobacteria, and Bacteroidetes, representing 64% of the total microbiota. Moreover, the Cai Rang River site (Ri-E), which is located at the merge point of wastewaters from backyard-based aquacultures, contained the genera Polynucleobacter, Variovorax, and Limnohabitans, representing more than 78.4% of the total microbiota. Bacterial diversity analysis showed that the Ri-E exhibited the lowest diversity compared with other regions. Principal coordinate analysis showed that the differences among water microbiotas in backyard-based aquacultures could be explained by the farmers' aquaculture techniques. In conclusion, this study demonstrated a collapse of bacterial diversity at the merge point of wastewaters from backyard-based aquacultures in the Mekong Delta.
Định danh: http://journal-dl.com/item/591088193fbb6e13743dd4a4
http://dspace.agu.edu.vn:8080/handle/AGU_Library/7083
Appears in Collections:Environment



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